PURIFICATION OF HYALURONIDASE

Purification and partial characterization of testicular hyaluronidase.

A new method is presented for the purification of testicular hyaluronidase involving ion exchange chromatography followed by gel filtration on Sephadex G-75. A highly purified hyaluronidase preparation has been obtained which contains 45,000 National Formulary activity units per mg, dry weight, and which migrates as a single component on polyacrylamide gel electrophoresis at pH 4.3. The enzyme ...

Heparin-sepharose affinity chromatography for purification of bull seminal-plasma hyaluronidase.

Bull seminal-plasma hyaluronidase was purified 180-fold by chromatography on concanvalin A-Sepharose, heparin Sepharose, Sephadex G-200 and Sephacryl S-200. With hyaluronic acid as the substrate, the specific activity and turnover number of purified hyaluronidase were 3.63 mumol/min per mg (104000 National Formulary units/mg of protein) and 214 min-1 (mol of product formed/mol of enzyme per min...

Crystal structure determination of hyaluronidase, a major bee venom allergen, in complex with an IgG Fab fragment and purification and biophysical characterization of bovine testes hyaluronidase

Staphylococcal hyaluronidase.

Introduction Plasma Hyaluronidase (Hyal-1) Genomic Organization of the Hyaluronidase Sequences Hyaluronidase Genes on Chromosome 3p21.3 Hyaluronidase Genes on Chromosome 7q31.3 Implications for Clinical Medicine Hyaluronidases in Cancer

he has focused on the biology of hyaluronan and the hyaluronidases. He is also a board-certified Anatomic Pathologist and participates in the teaching and diagnostic services of the Department of Pathology. He is currently a visiting scientist in the laboratory of Dr. Robert Stern in San Francisco. In addition to hyaluronan and hyaluronidase, his interests include the process of aging and the b...